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KMID : 0613820070170091298
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Journal of Life Science
2007 Volume.17 No. 9 p.1298 ~ p.1302
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β-lapachone Regulates Tight Junction Proteins, Claudin-3 and -4, in Human Hepatocarcinoma Cells
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Kim Sung-Ok
Kwon Jae-Im
Kim Ki-Young
Kim Nam-Deuk
Choi Yung-Hyun
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Abstract
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A hallmark of cancers is "leaky" tight junctions (TJs). TJs mediated paracellular permeability is elevated and TJs maintained cell polarity is frequently lost. Concomitantly, TJs-associated proteins including members of the claudin family of proteins are dysregulated. Recent findings indicate that these TJs changes can contribute to cancer progression. In this study, we examined the effects of β-lapachone, a quinone compound obtained from the bark of the lapacho tree (Tabebuia avellanedae), on the TJs-associated regulators in human hepatocarcinoma cell lines, HepG2 and Hep3B. β-lapachone treatment downregulated the levels of insulin-like growth factor 1 receptor (IGF-1R) proteins in both HepG2 and Hep3B cells. But the levels of claudin-3 and -4 proteins were increased in β-lapachone-treated HepG2 and Hep3B cells. And also the zonnula occludens-1 (ZO-1) and β-catenin protein levels by β-lapachone were increased in a time-dependent manner. However, claudin-3 and -4 mRNA levels were uninhibited by β-lapachone in HepG2 and Hep3B. The present results suggest that the upregulation of claudin-3 and -4 protein levels by β-lapachone occurs by a post-transcriptional mechanism and points to a novel mechanism by β-lapachone.
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KEYWORD
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β-lapachone, tight junction, IGF-1R, claudins, ZO-1, β-catenin
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